Forschungsthemen

Die Weiterentwicklung der Methoden und die Integration neuer Messverfahren ist ständiger Bestandteil der Arbeit im IDAS Dresden. Neben der Optimierung grundlegender Verfahren gibt es auch Substanzen, die besonders intensiv erforscht werden.

Die Schwerpunkte der Forschungsgebiete sind anhand unserer Publikationen ersichtlich:

 

2024

 

Insights into the metabolism of CH-PIATA - a novel synthetic cannabinoid featuring an acetamide linker

Zschiesche A, Scheu M, Thieme D, Keiler AM, Pulver B, Huppertz LM, Auwärter V

J Anal Toxicol. 2024 Mar 5:bkae013. doi: 10.1093/jat/bkae013

 

 

Elimination profile of low-dose chlortalidone and its detection in hair for doping analysis-Implication for unintentional non-therapeutic exposure

Thieme D, Weigel K, Anielski P, Krumbholz A, Sporkert F, Keiler AM.

Drug Test Anal. 2024 Feb 19. doi.org/10.1002/dta.3661

 

 

Endocannabinoids, endocannabinoid-like compounds and cortisone in head hair of health care workers as markers of stress and resilience during the early COVID-19 pandemic.

Biener I, Mueller TT, Lin J, Bao H, Steffen J, Hoerl M, Biere K, Matzel S, Woehrle T, König S, Keiler AM, Thieme D, Keppler O, Klein M, Weinberger T, Osterman A, Adorjan K, Choukér A.

Transl Psychiatry. 2024 Jan 31;14(1):71. doi: 10.1038/s41398-024-02771-9.

 

 

2023

 

QuEChERS as alternative extraction procedure in doping analyses.

Derwand D, Rzeppa S, Voss SC, Zschiesche A, Keiler AM.

Drug Test Anal. 2023 Dec 3. doi: 10.1002/dta.3610.

 

 

Extracellular Vesicles and Endocannabinoid Signaling in Patients with COVID-19.

Brandes F, Keiler AM, Kirchner B, Borrmann M, Billaud JN, Reithmair M, Klein M, Campolongo P,

Thieme D, Pfaffl MW, Schelling G, Meidert AS.

Cannabis Cannabinoid Res. 2023 Sep 14. doi: 10.1089/can.2023.0040.

 

 

Up to the maximum-testosterone dose-dependent effects on anabolic and androgen responsive tissues in orchiectomized rats.

Derwand D, Zierau O, Thieme D, Keiler AM.

Andrology. 2023 May 30. doi: 10.1111/andr.13466.

 

 

Identification of human in vitro metabolites of the haemoglobin S polymerization inhibitor voxelotor for doping control purposes.

Rzeppa S, Voss SC, Thieme D, Keiler AM.

Drug Test Anal. 2023 May 1. doi: 10.1002/dta.3489

 

 

Effect of HepG2 cell 3D cultivation on the metabolism of the anabolic androgenic steroid metandienone.

Gronert A, Zierau O, Thieme D, Keiler AM

Drug Test Anal. 2023 Feb 11. doi: 10.1002/dta.3455. Online ahead of print.

 

 

2022

 

Detection of 18-methyl steroids: Case report on a forensic urine sample and corresponding dietary supplements.

Thieme D, Anielski P, Rzeppa P, Wolf CA, Wolber G, Keiler AM

Drug Test Anal. 2022 Nov;14(11-12):1864-1870. doi: 10.1002/dta.3389. Epub 2022 Oct 26.

 

 

Medaka embryos as a model for metabolism of anabolic steroids.

Liu L, Hobohm L, Bredendiek F, Froschauer A, Zierau O, Parr MK, Keiler AM

Arch Toxicol. 2022 Jul;96(7):1963-1974. doi: 10.1007/s00204-022-03284-4.

 

 

Androgenic properties of the dietary supplement 5α-hydroxy-laxogenin.

Beer C, Keiler AM

Arch Toxicol. 2022 Jul;96(7):2139-2142. doi: 10.1007/s00204-022-03283-5.

 

 

Circulating inflammatory markers, cell-free mitochondrial DNA, cortisol, endocannabinoids, and N-acylethanolamines in female depressed outpatients.

Behnke A, Gumpp AM, Rojas R, Sänger T, Lutz-Bonengel S, Moser D, Schelling G, Krumbholz A, Kolassa IT

World J Biol Psychiatry. 2022 May 9:1-12. doi: 10.1080/15622975.2022.2070666

 

 

Application of single hair analysis in a doping case involving amphetamine

Wiedfeld C, Skopp G, Thieme D, Musshoff F

Drug Test Anal. 2022 Apr;14(4):781-784. doi: 10.1002/dta.3217

 

 

B-cell homeostasis is maintained during two months of head-down tilt bed rest with or without antioxidant supplementation.

Bonnefoy J, Baselet B, Moser D, Ghislin S, Ferreira Da Silva Miranda S, Riant E, Vermeesen R, Keiler AM, Baatout S, Choukér A. Frippiat J-P

Front Immunol. 2022 Feb 16. Doi: 10.3389/fimmu.2022.830662.

 

 

HepG2 as promising cell-based model for biosynthesis of long-term metabolites - exemplified Metandienone.

Zschiesche A, Chundela Z, Thieme D, Keiler AM

Drug Test Anal. 2022 Feb;14(2):298-306. doi: 10.1002/dta.3184.

 

 

2021

 

Emergence of the less common cannabinoid Δ8 -Tetrahydrocannabinol in a doping sample.

Rzeppa S, Große J, Rautenberg C, Thieme D, Lepp T, Vallimäe H, Keiler AM

Drug Test Anal. 2021 Nov;13(11-12):1936-1943. doi: 10.1002/dta.3159.

 

New insights into the metabolism of methyltestosterone and metandienone: Detection of novel A-ring reduced metabolites.

Loke S, Liu L, Wenzel M, Scheffler H, Iannone M, de la Torre X, Schlörer N, Botrè F, Keiler AM, Bureik M, Parr MK

Molecules 2021, Mar 3 26(5), 1354. doi.org/10.3390/molecules26051354.

 

 

Cell culture as a toolbox to generate phase I metabolites for antidoping screening.

Savill R, Baues H, Voigt E, Zierau O, Thieme D, Keiler AM

Drug Test Anal. 2021 Jun;13(6):1169-1177. doi: 10.1002/dta.3009.

 

 

Hair-based biomarkers in women with major depressive disorder: Glucocorticoids, endocannabinoids, N-acylethanolamines, and testosterone.

Behnke A, Gumpp AM, Krumbholz A, Bach AM, Schelling G, Kolassa IT, Rojas R

Compr Psychoneuroendocrinol. 2021 Jun 21;7:100068. doi: 10.1016/j.cpnec.2021.100068

 

 

Semen quality and testicular adrenal rest tumour development in 46,XY congenital adrenal hyperplasia: the importance of optimal hormonal replacement.

Rohayem J, Bäumer LM, Zitzmann M, Fricke-Otto S, Mohnike K, Gohlke B, Reschke F, Jourdan C, Müller HL, Dunstheimer D, Weigel J, Jorch N, Müller-Rossberg E, Lankes E, Gätjen I, Richter-Unruh A, Hauffa BP, Kliesch S, Krumbholz A, Brämswig J

Eur J Endocrinol. 2021 Apr;184(4):487-501. doi: 10.1530/EJE-20-1154

 

 

2020

 

Exploring the Utility of Hair Endocannabinoids for Monitoring Homeostasis in Bonobos.

Behringer V, Krumbholz A, Stevens JMG, Keiler AM, Zierau O, Hohmann G

Physiol Biochem Zool. Mar-Apr 2021;94(2):83-98. doi: 10.1086/712658.

 

 

Associating Emergency Medical Services personnel's workload, trauma exposure, and health with the cortisol, endocannabinoid, and N-acylethanolamine concentrations in their hair.

Behnke A, Karabatsiakis A, Krumbholz A, Karrasch S, Schelling G, Kolassa IT, Rojas R

Sci Rep. 2020 Dec 29;10(1):22403. doi: 10.1038/s41598-020-79859-x

 

 

Detection of the selective androgen receptor modulator GSK2881078 and metabolites in urine and hair after single oral administration.

Rading A, Anielski P,Thieme D, Keiler AM

Drug Test Anal. 2021 Jan;13(1):217-222. doi: 10.1002/dta.2943.

 

 

Chronic Exposure to Low-Dose Carbon Monoxide Alters Hemoglobin Mass and V?O2max.

Schmidt WFJ, Hoffmeister T, Haupt S, Schwenke D, Wachsmuth NB, Byrnes WC

Med Sci Sports Exerc. 2020 Sep;52(9):1879-1887

 

 

2019

 

Comparison of the three SARMs RAD 140, GLPG0492 and GSK 2881078 in two different in vitro bioassays, and in an in silico androgen receptor binding assay.

Zierau O, Kolodziejczyk A, Vollmer G, Machalz D, Wolber G, Thieme D, Keiler AM

J Steroid Biochem Mol Biol. 2019 May;189:81-86. https://doi: 10.1016/j.jsbmb.2019.02.014.

 

 

Stress Related Shift Toward Inflammaging in Cosmonauts After Long-Duration Space Flight.

Buchheim JI, Matzel S, Rykova M, Vassilieva G, Ponomarev S, Nichiporuk I, Hörl M, Moser D, Biere K, Feuerecker M, Schelling G, Thieme D, Kaufmann I, Thiel M, Choukèr A

Front Physiol. 2019 Feb 19;10:85. doi: 10.3389/fphys.2019.00085

 

 

Steroid 17-Hydroxyprogesterone in Hair Is a Potential Long-Term Biomarker of Androgen Control in Congenital Adrenal Hyperplasia due to 21-Hydroxylase Deficiency

Auer MK, Krumbholz A, Bidlingmaier M, Thieme D, Reisch N

Neuroendocrinology. 2020;110(11-12):938-949. doi: 10.1159/000504672

 

 

2018

 

Influence of estrogen on individual exercise motivation and bone protection in ovariectomized rats.

Müller ST, Keiler AM, Kräker K, Zierau O, Bernhardt R

Lab Anim. 2018 Oct;52(5):479-489. doi: 10.1177/0023677218756455

 

 

Erythropoietic effects of low-dose cobalt application.

Hoffmeister T, Schwenke D, Wachsmuth N, Krug O, Thevis M, Byrnes WC, Schmidt WFJ

Drug Test Anal. 2019 Feb;11(2):200-207. doi: 10.1002/dta.2478

 

 

In vitro metabolism of selected bioactive compounds of Eurycoma longifolia root extract to identify

suitable markers in doping control.

Bräuer P, Anielski P, Schwaiger S, Stuppner H, Tran TVA, Vollmer G, Zierau O, Thieme D, Keiler AM

Drug Test Anal. 2019 Jan;11(1):86-94. Epub 2018 Aug 9.

 

 

Determination of hydroxy metabolites of cocaine in hair samples for proof of consumption.

Musshoff F, Thieme D, Schwarz G, Sachs H, Skopp G, Franz T

Drug Test Anal. 2018 Apr;10(4):681-688  . doi: 10.1002/dta.2317

 

 

Effects of 3 Weeks of Oral Low-Dose Cobalt on Hemoglobin Mass and Aerobic Performance.

Hoffmeister T, Schwenke D, Krug O, Wachsmuth N, Geyer H, Thevis M, Byrnes WC, Schmidt WFJ

Front Physiol. 2018 Sep 19;9:1289. doi: 10.3389/fphys.2018.01289

 

 

The plasma protein binding of the endogenous glucocorticosteroids is of vital importance for the concentrations in hair and saliva.

Krumbholz A, Schönfelder M, Hofmann H, Thieme D

Forensic Sci Int. 2018 May;286:23-30      10.1016/j.forsciint.2018.01.030

 

 

Modulations of Neuroendocrine Stress Responses During Confinement in Antarctica and the Role of Hypobaric Hypoxia.

Strewe C, Thieme D, Dangoisse C, Fiedel B, van den Berg F, Bauer H, Salam AP, Gössmann-Lang P, Campolongo P, Moser D, Quintens R, Moreels M, Baatout S, Kohlberg E, Schelling G, Choukèr A, Feuerecker M

Front Physiol. 2018 Nov 26;9:1647. doi: 10.3389/fphys.2018.01647

 

 

Androgen- and estrogen-receptor mediated activities of 4-hydroxytestosterone, 4-hydroxyandrostenedione and their human metabolites in yeast based assays.

Keiler AM, Zierau O, Wolf S, Diel P, Schänzer W, Vollmer G, Machalz D, Wolber G, Parr MK

Toxicol Lett. 2018 Aug; 292:39-45. Epub 2018 Apr 24.